Coding

Part:BBa_Y00012:Design

Designed by: Natalie Kuldell   Group: Yeast-1   (2006-07-06)


mitochondrial LYS12


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 9
    Illegal suffix found in sequence at 1147
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 9
    Illegal SpeI site found at 1148
    Illegal PstI site found at 1162
    Illegal NotI site found at 15
    Illegal NotI site found at 1155
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 9
    Illegal BamHI site found at 1069
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 9
    Illegal suffix found in sequence at 1148
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 9
    Illegal XbaI site found at 24
    Illegal SpeI site found at 1148
    Illegal PstI site found at 1162
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 399


Design Notes

codon optimized for expression in S. cerevisiae mitochondria, keeping all biobrick sites out of coding region


Source

custom synthesis from DNA 2.0

References